Bovine alpha Lactalbumin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

alpha Lactalbumin

Applications

Competitive ELISA

Reactivity

Bovine

Assay type

Competitive (quantitative)

Sensitivity

1.875 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for alpha Lactalbumin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

3.125-200 ng/ml

Assay Time

1 hour

Principiul testului

Bovine alpha Lactalbumin ELISA Kit (A87162) is a 1 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine alpha Lactalbumin in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for alpha Lactalbumin. During the assay, alpha Lactalbumin present in the standards or samples competes with HRP-labelled alpha Lactalbumin for binding to the immobilized capture antibody. Following incubation, unbound components are removed by washing and TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of alpha Lactalbumin in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.