Anti-Vinculin Antibody [VCL/3617]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Vinculin

Applications

IF, Flow Cytometry, IHC-P

Reactivity

Human, Mouse, Rat, Bovine, Porcine, Rabbit, Frog, Fish, Bird

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

IF: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, IHC-P: 1-2 µg/ml

Molecular weight

125-135 kDa

Light chain

kappa

Clonă

VCL/3617

Imunogen

Recombinant full-length human Vinculin protein.

Specificitate

This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului