Anti-uPA Receptor / U-PAR Antibody [VIM-5]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

uPA Receptor/U-PAR

Applications

IP, Flow Cytometry, IHC-Fr

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

VIM-5

Imunogen

THP-1 cells (human acute monocytic leukaemia cells).

Specificitate

This antibody recognises human CD87, also known as urokinase plasminogen activator receptor (uPAR), a 50-65 kDa single chain glycosylphostidylinsitol (GPI) anchored glycoprotein, expressed by granulocytes, tissue macrophages, monocytes, endothelial cells, fibroblasts and mesenchymal and epithelial tumour cells. CD87 specifically binds and activates urokinase plasminogen activator (uPA), a serine protease which converts the inactive receptor-bound proenzyme plasminogen into plasmin, triggering the proteolysis cascade of fibrinolysis. The interaction between uPAR and uPA is also known to play a role in cancer cell invasion and metastasis. Clone VIM-5 is reported to block urokinase binding.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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