Anti-UBE2B Antibody [PCRP-UBE2B-1C7] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Ube2B

Applications

Flow Cytometry, IF

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Molecular weight

17 kDa

Cross reactivity

This antibody is predicted to cross react with Mouse, Rat, Xenopus, and Zebrafish.

Clonă

PCRP-UBE2B-1C7

Imunogen

Recombinant full-length human UBE2B protein.

Specificitate

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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