Anti-Tyrosine Hydroxylase (phospho Ser40) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µl

Țintă

Tyrosine Hydroxylase

Applications

WB, IHC-Fr, IF

Reactivity

Rat, Mouse, Human, Pig

Formulation

Supplied in 10mM HEPES, pH 7.5, with 0.01% BSA, 50% Glycerol, and 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

IF: 1:1,000, IHC-Fr: 1:1,000, WB: 1:1,000

Molecular weight

Approximately 60 kDa in PC-12 cell lysates, following stimulation by Okadaic acid.

Imunogen

Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes the phosphorylated residue Ser 40.

Specificitate

This antibody recognises tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, when phosphorylated at serine 40.Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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