Anti-Transferrin Receptor Antibody [OX-26] - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

Transferrin Receptor

Applications

Flow Cytometry, WB, IHC-Fr, IHC-P, IF

Reactivity

Rat

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1:50 for 1 mg, 0.1 mg, 1:10 for 0.25 mg - 1:200 for 1 mg, 0.1 mg, 1:100 for 0.25 mg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

OX-26

Imunogen

PHA activated rat lymphocytes.

Specificitate

This antibody recognises rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009). Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy. OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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