Anti-RNA Polymerase II CTD repeat YSPTSPS Antibody [CTD4H8] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

RNA polymerase II CTD repeat YSPTSPS

Applications

Flow Cytometry, IF, WB, IHC-P

Reactivity

Human, Mouse, Rat

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

192-253 kDa

Light chain

kappa

Clonă

CTD4H8

Imunogen

Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho Ser5.

Specificitate

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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