Anti-Rel B Antibody [17.3]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

Rel B

Applications

WB, IHC-P, IP, IF, ICC

Reactivity

Human, Rhesus Monkey, Mouse

Formulation

Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

500 µg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ICC: 1 µg/ml, IF: 1 µg/ml, IHC-P: 1:10 - 1:50, WB: 1 - 2 µg/ml

Molecular weight

Approximately 63 kDa in THP-1 cell lysate, Daudi cell lysate, and Raji cell lysate

Clonă

17.3

Imunogen

Recombinant protein derived from C-terminus of human RelB protein.

Specificitate

This antibody recognises the transcription factor RelB, also known as I-Rel. RelB is encoded by the v-rel avian reticuloendotheliosis viral oncogene homolog B gene and is a critical component of the NF-kappa-B pathway.Upon cleavage of the NF-kappa-B p105 and p100 subunits into the NF-kappa-B p50 and p52 subunits, RelB forms RelB-p50 and RelB-p52 heterodimers. These dimers act as activators of transcription by binding to kappaB sites present on DNA. RelB-p50 and RelB-p52 binding induces the expression of genes mediating immune responses, inflammation and apoptosis. Misregulation of the NF-kappa-B pathway has been implicated in a variety of disease states including autoimmunity and cancer. As the NF-kappa-B pathway has been demonstrated to be constitutively active in a large group of tumors, developing NF-kappa-B inhibitors has emerged as a new cancer therapy approach (Chaturverdi et al. 2011 and Baud and Karin et al. 2009).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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