Anti-Rad9 Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

Rad9

Applications

WB, ELISA 

Reactivity

S. cerevisiae

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

500 µg/ml

Isotype

IgG

Recommended dilutions

ELISA: 1:2,000 - 1:4,000, WB: 1:50 - 1:250

Molecular weight

Approximately 60 kDa.

Protein Length

15

Secvență

PSGESFRLSLDSPSK

Imunogen

Synthetic peptide corresponding to amino acids 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin.

Aminoacizi

aa 1125 - 1139

Specificitate

This antibody recognises Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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