Anti-Perforin Antibody [dG9]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Perforin

Applications

Flow Cytometry, IHC-Fr, IP, IHC-P, ICC

Reactivity

Human, Bovine

Formulation

Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Cross reactivity

This antibody does not cross-react with Mouse.

Purity

> 95% (by SDS-PAGE).

Clonă

dG9

Imunogen

Purified granules from human YT lymphoma cell line.

Specificitate

This antibody recognises perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation. Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias. This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs.

Storage

Store undiluted at 4°C. Do not freeze!

Documentație

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