Anti-PCNA Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

PCNA

Applications

WB, IHC-P, ELISA

Reactivity

Human, Chicken, Fish, Rat, Amphibia, Bovine, Monkey, Mouse, Dog

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

500 µg/ml

Isotype

IgG

Recommended dilutions

ELISA: 1:2,000 - 1:8,000, IHC-P: 2.5 µg/ml, WB: 1:500 - 1:2,000

Imunogen

Synthetic peptide corresponding to the internal region of human PCNA protein.

Specificitate

This antibody recognises human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36 kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair. PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohistochemistry on paraffin sections using rabbit anti human PCNA antibody.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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