Anti-Nestin Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

Nestin

Applications

WB, IHC-P, ELISA

Reactivity

Human, Macaque, Mouse, Bovine, Dog, Hamster, Rat, Rabbit

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

500 µg/ml

Isotype

IgG

Recommended dilutions

ELISA: 1:20,000 - 1:80,000, IHC-P: 5 µg/ml, WB: 1:500 - 1:3,000

Protein Length

17

Secvență

PILTDAHELQPQAEGIQ

Imunogen

Recombinant human Nestin (amino acids 1484-1500).

Aminoacizi

aa 1484 - 1500

Specificitate

This antibody recognises human Nestin, an intermediate filament involved in cytoskeletal organisation.Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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