Anti-N Cadherin Antibody [8C11] - Equivalent to Abcam (ab19348)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

N Cadherin

Applications

Flow Cytometry, IF, WB, IHC-P

Reactivity

Human, Mouse

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

130-140 kDa

Light chain

kappa

Clonă

8C11

Imunogen

Recombinant human N Cadherin cytoplasmic domain.

Specificitate

This antibody recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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