Anti-Myeloperoxidase Antibody [D08-8G5] (Biotin)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Myeloperoxidase

Applications

ELISA

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

Biotin

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 1 µg/ml.

Clonă

D08-8G5

Imunogen

Purified native human myeloperoxidase.

Specificitate

This antibody recognises myeloperoxidase (MPO), a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is largely expressed by neutrophils within azurophilic granules, but is also present in monocytes, macrophages and central nervous microglial cells. MPO is synthesized as a precursor protein that undergoes a series of modifications including cleavage and glycosylation to form the catalytically inactive MPO precursor. Subsequently an iron-heme is incorporated into the protein resulting in the catalytically active MPO. In response to bacterial infection, neutrophils containing MPO fuse with the phagosome and when the common membrane is ruptured, MPO is release into the phagosome. MPO then uses H2O2 to generate the bactericidal compound, hypochlorous acid (HClO). MPO deficiency is associated with a higher occurrence of severe infections and chronic inflammatory processes, although ~50% of individuals are asymptomatic (Antachopoulos 2010).The biotinylated Mouse anti Human myeloperoxidase antibody, clone D08-8G5 (MCA6261B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human myeloperoxidase antibody, clone D02-2A1 (MCA6260GA) as the capture antibody.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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