Anti-Myelin Basic Protein Antibody [7G7]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

10µl · 50µl · 100µl

Țintă

Myelin Basic Protein

Applications

WB, ICC/IF, IHC

Reactivity

Human, Horse, Bovine, Porcine, Rat, Mouse

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Immunogen affinity purification.

Isotype

IgG1

Recommended dilutions

WB: 1:10,000-1:20,000, ICC/IF: 1:2,000-1:5,000, IHC: 1:2,000-1:10,000

Molecular weight

Rodents: 14 kDa, 17 kDa, 18.5 kDa, 21.5 kDa. Human: 17.2 kDa, 18.5 kDa, 20.5 kDa, 21.5 kDa.

Light chain

kappa

Clonă

7G7

Imunogen

Native Myelin Basic Protein isolated and purified from bovine brain.

Specificitate

This antibody binds all four of the CNS Myelin Basic Protein isoforms; with the epitope mapped to peptide TPPPSQGKG, amino acids 125-133 of the human 21.5 kDa sequence, with the last four amino acids, SQGKG, likely to be key elements of the epitope. This antibody can be used to identify oligodendrocytes and Schwann cells in neural cell culture, to visualize myelin sheaths and myelinating cells in sectioned material, and to probe western blots for MBP gene products. This antibody is also rather insensitive to aldehyde fixation and therefore can be used in immunohistochemistry of paraffin sections.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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