Anti-MUC1 Antibody [SPM492]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

MUC1

Applications

ELISA, Flow Cytometry, IF, IHC-P

Reactivity

Human, Mouse

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

ELISA: 2-4 µg/ml, Flow Cytometry: 0.25-0.5 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

265-400 kDa

Light chain

kappa

Clonă

SPM492

Imunogen

Breast Cancer Cell line ZR75-1.

Specificitate

This antibody reacts with MUC1. The dominant epitope of This antibody has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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