Anti-MMP9 Antibody [I13-2E1]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

MMP9

Applications

ELISA

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 1 µg/ml.

Clonă

I13-2E1

Imunogen

E. coli derived recombinant fragment of human MMP-9 (Gly373-Pro456).

Specificitate

This antibody recognises matrix metalloproteinase 9 (MMP-9) also known as 92 kDa gelatinase and 92 kDa type IV collagenase. MMP-9 is a member of the zinc-dependent endopeptidases and is expressed in various cell types, including neutrophils, macrophages, fibroblasts and endothelial cell (Huang 2018). Expression of MMP-9 is induced by pro-inflammatory cytokines and is synthesized as a pre-proenzyme with a 19 amino acid N-terminal signal peptide. It can then be secreted by the cell into the extracellular environment where it is modified into the inactive pro-MMP9. At this point it can be activated by various proteases such as MMP-3, to generate the activated 82 kDa form. Activated MMP-9 can proteolytically cleave many extracellular matrix proteins including gelatin, collagen and elastin as part of extracellular matrix remodeling.Overexpression of MMP-9 has been observed in a range of malignant tumors and its’ extracellular matrix remodeling has been linked with mediating tumor invasion, metastasis, angiogenesis and regulating the tumor microenvironment (Huang 2018). MMP-9 has also been associated with other pathologies including autoimmune and cardiovascular diseases.The purified Mouse anti Human MMP-9 antibody, clone I13-2E1 (MCA6196GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human MMP-9 antibody, clone B10-7B9 (MCA6197B) as the detection antibody.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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