Anti-MMP2 Antibody [C12-8A3]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

MMP2

Applications

ELISA

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

ELISA: 1 µg/ml.

Clonă

C12-8A3

Imunogen

E. coli derived recombinant human partial length of MMP-2 (Tyr110-Thr465).

Specificitate

This antibody recognises matrix metalloproteinase-2 also known as 72 kDa type IV collagenase, 72 kDa gelatinase and gelatinase A. MMP-2 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells, adventitia platelets and leukocytes. MMP-2 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 72 kDa pro-MMP-2. Pro-MMP-2 is then recruited to the cell surface where it undergoes autocatalytic cleavage to form the active 63 kDa form.Like other MMP’s, MMP-2 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-2 degrades the collagen proteins, type I, II, III, IV, V, VII, X, XI and gelatin. It is also able to target other ECM substrates including aggrecan, elastin, fibronectin and laminin. However a number of non-matrix proteins have been identified as targets of MMP-2, to regulate various physiological and pathophysiological processes including embryonic growth/development, angiogenesis, vascular diseases, inflammation, infective diseases and tumor progression. MMP-2 may also play a role in airway remodeling and may be responsible for the progressive decline of lung function in bronchial asthma. The purified Mouse anti Human MMP-2 antibody, clone C12-8A3 (MCA6256GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human MMP-2 antibody, clone J08-3B10 (MCA6257B) as the detection antibody.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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