Anti-MiTF Antibody [PCRP-MITF-1D9]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

MiTF

Applications

IP, IF, Flow Cytometry, WB

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2b

Recommended dilutions

IP: 1-2µg / 100-500µg proteins, IF: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, WB: 1-2 µg/ml

Molecular weight

52-56 kDa (doublet)

Cross reactivity

This antibody is predicted to cross react with Chicken, Mouse, Rat, Xenopus, and Zebrafish.

Clonă

PCRP-MITF-1D9

Imunogen

Recombinant full-length human MiTF protein.

Specificitate

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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