Anti-Melanoma gp100 Antibody [HMB45 + PMEL/783]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Melanoma gp100

Applications

WB, IHC-P

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Oligoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1, kappa + IgG1, kappa

Recommended dilutions

WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

26-100 kDa

Clonă

HMB45 + PMEL/783

Imunogen

Clone HMB45: Extract of pigmented melanoma metastases from lymph nodes. Clone PMEL/783: Recombinant human SILV protein.

Specificitate

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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