Anti-MCP1 Antibody [2.2-4H5-1A11] - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

MCP1

Applications

WB, ELISA, IHC-P

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 1.0 - 2 µg/ml, IHC-P: 10 µg/ml, WB: 0.2 - 0.4 µg/ml

Reconstitution

Reconstitute with 0.5 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Clonă

2.2-4H5-1A11

Imunogen

Recombinant human MCP-1/MCAF.

Specificitate

This antibody recognises human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13 kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils. MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells. MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype. Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection. recognises both native and recombinant MCP-1 in conjunction with Rabbit anti Human MCP-1 polyclonal antibody in a sensitive sandwich ELISA assay.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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