Anti-Integrin beta 1 Antibody [12G10]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 200µg

Țintă

Integrin beta 1

Applications

Flow Cytometry, IHC-Fr, ELISA, IF

Reactivity

Human, Mink, Rabbit

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 10 µg/ml, Flow Cytometry: 1:25 - 1:50

Cross reactivity

This antibody does not cross react with Rat or Mouse

Clonă

12G10

Imunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.

Specificitate

This antibody recognises human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions. Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes. Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments. Binding of antibody clone 12G10 to the integrin ß1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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