Anti-Integrin alpha 4 / CD49D Antibody [HP2/1]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

100µg · 200µg

Țintă

Integrin alpha 4/CD49D

Applications

IP, Flow Cytometry, IHC-Fr, Functional Assays

Reactivity

Human, Rat, Rhesus Monkey, Bovine, Pig, Cynomolgus monkey, Goat, Rabbit, Llama, Horse, Mink, Mustelid, Cat

Formulation

Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1ug/5 x 105 cells

Clonă

HP2/1

Imunogen

JM leukaemia line.

Specificitate

This antibody recognises human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria. Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells. Mouse anti Human CD49d, clone HP2/1 can be used in assays of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and inhibits cell binding to soluble VCAM-1.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului