Anti-IL-8 Antibody (Biotin) - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

IL-8

Applications

WB, ELISA 

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Biotin

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Imunogen

Recombinant Human IL-8.

Specificitate

This antibody recognises human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils.IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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