Anti-IL-4R Antibody [25463 (49/20)]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

IL-4R

Applications

IHC-P, WB, ELISA, Flow Cytometry, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.5% Trehalose.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

500 µg/ml

Purification

Protein A affinity chromatography of ascites.

Isotype

IgG2a

Recommended dilutions

ELISA: 1:250 - 1:1,000, Flow Cytometry: 1:10 - 1:40, IHC-P: 1:20 - 1:60, WB: 1:125 - 1:500

Clonă

25463 (49/20)

Imunogen

Spodoptera frugiperda lepidopteran ovarian cell line Sf21-derived, recombinant human interleukin 4 receptor, extracellular domain.

Specificitate

This antibody recognises human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kDa type I single pass transmembrane glycoprotein bearing a single fibronectin type III domain. Interleukin-4 Receptor form a heterodimeric complex with the cytokine receptor common subunit gamma, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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