Anti-IL-2 Receptor alpha Antibody [P4A10] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

IL-2 Receptor alpha

Applications

Flow Cytometry

Reactivity

Canine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

P4A10

Imunogen

Enriched and stimulated Canine T cells.

Specificitate

This antibody recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ra, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes. The IL-2 receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132), CD25 functions as a low affinity receptor for IL-2.Antibodies to CD4,Mouse anti Human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25. However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody. The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers , and in genetically related diseases of the hemopoietic system (Bauer Jr.et al. 2009).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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