Anti-IGF1 Antibody (Biotin) - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

IGF1

Applications

WB, ELISA 

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Biotin

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

100 µg/ml after reconstituiton.

Isotype

IgG

Recommended dilutions

ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Imunogen

Recombinant human IGF-I.

Specificitate

This antibody recognises human IGF-I (Insulin-like Growth Factor I), a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth.IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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