Anti-ICAM1 Antibody [84H10]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 200µg

Țintă

ICAM1

Applications

ELISA, Functional Assays, Flow Cytometry, IHC-Fr, IP

Reactivity

Human, Dog

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat - 1:10, IHC-Fr 2: 1:20 - 1:50, IP: 5µg per 107 cells, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

84H10

Imunogen

K562 cell line.

Specificitate

This antibody recognises the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Mouse anti Human CD54 antibody, clone 84H10 detects an antigen of ~90 kDa. Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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