Anti-HMGB1 Antibody [HMG1-5H6]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

HMGB1

Applications

WB, IF, Flow Cytometry

Reactivity

Mouse, Rat, Human, Hamster

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Hamster

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography.

Isotype

IgG

Recommended dilutions

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Molecular weight

Approximately 30 kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30 kDa (red) and 27 kDa (ox) in the absence of 2ME.

Clonă

HMG1-5H6

Imunogen

Recombinant mouse HMGB1.

Specificitate

This antibody recognises high mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein belonging to the alarmin family. HMGB1 is released by necrotic and inflammatory cells acting as a chemoattractant for immature dendritic cells (DCs) and promoting their maturation. DCs can also secrete HMGB1, leading to proliferation and Th1 polarization of interacting T cells. HMGB1 is angiogenic and promotes cardiac stem cell growth and differentiation, it is a damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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