Anti-HLA DRB Antibody [HLA-DRB/1067]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

HLA DRB

Applications

Flow Cytometry, IF, WB, IHC-P

Reactivity

Human, Monkey

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-4 µg/ml, IHC-P: 0.25-0.5 µg/ml

Molecular weight

28 kDa (beta chain)

Light chain

kappa

Clonă

HLA-DRB/1067

Imunogen

Activated human peripheral blood mononuclear cells.

Specificitate

This antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of MAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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