Anti-Histone H4 (acetyl Lys 5) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H4

Applications

ChIP, WB, IF

Reactivity

Human, Mouse, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 5 µg per ChIP, IF: 1:500, WB: 0.5 µg/ml - 2 µg/ml

Imunogen

A peptide including acetylated lysine 5 of human histone H4.

Specificitate

This antibody recognises Histone H4 when acetylated at lysine 5.Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of histone H4 usually results in transcriptional activation. Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac5) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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