Anti-Golgi Complex Antibody [AE-6]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Golgi Complex

Applications

Flow Cytometry, IF, WB, ICC, IHC-P

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, ICC: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Cross reactivity

This antibody does not cross-react with Mouse or Rat.

Light chain

kappa

Clonă

AE-6

Imunogen

SU-DHL-1 large cell lymphoma cells.

Specificitate

This antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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