Anti-GLG1 Antibody [GLG1/970] (CF® 488)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

GLG1

Applications

Flow Cytometry, IF

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

CF®488

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Molecular weight

134 kDa

Cross reactivity

This antibody does not cross-react with Mouse or Rat.

Light chain

kappa

Clonă

GLG1/970

Imunogen

Golgi fraction from human liver cells.

Specificitate

This antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. This antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Storage

Store at 4-8°C. Antibody is stable for 24 months.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului