Anti-GAD65 Antibody [N-GAD65]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

GAD65

Applications

WB, IHC-P, IP, IHC-Fr, RIA

Reactivity

Human, Rat, Monkey

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

WB: 1:100-1:1,000

Protein Length

19

Clonă

N-GAD65

Secvență

PGSGFWSFGSEDGSGDSEN

Imunogen

Synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminus of human GAD65, conjugated to Keyhole Limpet Haemocyanin.

Aminoacizi

aa 4 - 22

Specificitate

This antibody recognises human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of ?-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system and is expressed in the brain and pancreatic beta cells.Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease , Stiff Man Syndrome and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles. recognises GAD65 but does not recognize GAD67.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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