Anti-G-CSF Antibody - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

G-CSF

Applications

WB, ELISA, IHC-P, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.5 µg/ml - 2 µg/ml, Functional Assays: 0.04 - 0.06 µg/ml, IHC-P: 0.25 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Imunogen

Recombinant Human G-CSF.

Specificitate

This antibody recognises human G-CSF (Granulocyte colony-stimulating factor), a 19.6kDa haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage.The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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