Anti-Estrogen Receptor alpha Antibody [6F11]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

100µl · 1mL

Țintă

Estrogen Receptor alpha

Applications

WB, IHC-P, IHC-Fr

Reactivity

Human

Formulation

Supplied in 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG1

Recommended dilutions

IHC-Fr 1: 1:40 - 1:60, IHC-P 2: 1:40 - 1:80, WB: 1:50 - 1:100

Purity

Tissue culture supernatant.

Clonă

6F11

Imunogen

Recombinant human estrogen receptor (alpha form).

Specificitate

This antibody recognises the human estrogen receptor alpha chain (ERa), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERa is a ~65 kDa steroid hormone receptor containing an N-terminal (AF-1) ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an (AF-2) domain. ERa binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy. Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERa on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERa in breast cancer tissues.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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