Anti-ERK2 Antibody [PCRP-MAPK1-1D1] - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

ERK2

Applications

ELISA, IP, Flow Cytometry, IF

Reactivity

Human

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2b

Recommended dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 0.5-2 µg/million cells, IF: 0.5-2 µg/ml

Molecular weight

41.3 kDa

Clonă

PCRP-MAPK1-1D1

Imunogen

Recombinant full-length human MAPK1 protein.

Specificitate

Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului