Anti-Endothelin 1 Antibody [IC4]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Endothelin 1

Applications

IHC-Fr, IRMA

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Clonă

IC4

Imunogen

C-terminus fragment of human endothelin 1.

Specificitate

This antibody recognises human endothelin 1 (ET-1). Endothelin-1 in it's precursor form is a 212 amino acid protein with a 17 aa signal peptide, an N-terminal pro-peptide region and a C-terminal pro-peptide region flanking the endothelin peptides, big endothelin-1 (38aa), the precursor of the smaller endothelin peptide which is cleaved by endothlin converting enzyme to produce the active endothelin-1 (21aa) peptide and a C-terminal fragment. Endothelin-1 is a potent vasoconstrictor with a molecular mass of approximately 25 kDa. Mouse anti human endothelin 1 antibody, clone IC4 shows less than 10% reactivity with endothelin 3.Plasma levels of both endothlin-1 and big endothlin-1 are useful as predictors for survival of patients with congestive heart failure. However, the half life of the endothlin1 peptide in human plasma is very short (c1.0 mins) and measurement of big-endothlin-1 may be a more reliable measure of the endothlin secretory pathway activity (Hemsén et al. 1995).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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