Anti-EGF Antibody - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

EGF

Applications

WB, ELISA, IHC-P, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.5 - 2 µg/ml, Functional Assays: <0.1 µg/ml, IHC-P: 5.0 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Imunogen

Recombinant human EGF.

Specificitate

This antibody recognises human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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