Anti-E Cadherin Antibody [67A4]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

E Cadherin

Applications

Flow Cytometry, IHC-Fr, IP, WB, ICC

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:10 - 1:20, IHC-Fr: 1:125 - 1:250, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Purity

> 95% (by SDS-PAGE).

Clonă

67A4

Imunogen

T-47D cells.

Specificitate

This antibody recognises CD324 also known as E-Cadherin, a transmembrane glycoprotein which mediates calcium dependant cell adhesion and cell junction formation. The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions. The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells. This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).

Storage

Store undiluted at 4°C. Do not freeze!

Documentație

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