Anti-DYKDDDDK Tag Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

50µg · 100µg

Țintă

DYKDDDDK Tag

Applications

WB, ELISA 

Reactivity

Species independent

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

500 µg/ml

Isotype

IgG

Recommended dilutions

ELISA: 1:9,000 - 1:450,000, WB: 1:2,000 - 1:10,000

Secvență

DYKDDDDK

Imunogen

Synthetic peptide sequence DYKDDDDK corresponding to the enterokinase cleavage site, conjugated to Keyhole Limpet Haemocyanin. Residues of cysteine and glycine were used to facilitate coupling at the C-terminus end.

Specificitate

This antibody recognises the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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