Anti-Dectin-1 Antibody [2A11] (PE)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25T · 100T

Țintă

Dectin-1

Applications

Flow Cytometry

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

PE

Host

Rat

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Reconstitution

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Clonă

2A11

Imunogen

Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.

Specificitate

This antibody recognises mouse beta-glucan receptor, also known as Dectin-1 or CD369. Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului