Anti-Cytomegalovirus gB Late Cytoplasmic Antigen Antibody [hCMV34]

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Descriere

Specificații

Brand

Antibodies

Mărime

200µg

Țintă

Cytomegalovirus gB Late Cytoplasmic Antigen

Applications

ELISA, IHC-Fr, IF

Reactivity

Viral

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

IF: 0.02 µg/slide - 1 µg/slide

Clonă

hCMV34

Imunogen

Native cytomeglaovirus extracts from infected human cells.

Specificitate

This antibody recognises cytomegalovirus glycoprotein B late cytoplasmic antigen. Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of approximately 100kDa. Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen antibody, clone hCMV34 does not react with HSV, VZV or EBV. Reacts with MRC-5 infected cells, but not uninfected cells. Mouse anti Cytomegalovirus gB Late Cytoplasmic Antigen antibody, clone hCMV34 has been used successfully to demonstrate presence of CMV in infected cells by immunofluorescence on paraformaldehyde fixed cell cultures (González-Sánchez et al 2015).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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