Anti-Cystatin C Antibody [B01-8B1]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Cystatin C

Applications

ELISA

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 1 µg/ml.

Clonă

B01-8B1

Imunogen

E. coli derived recombinant human full length cystatin C (Ser27-Ala146).

Specificitate

This antibody recognises cystatin C, a small non-glycosylated 13.3 kDa basic cysteine inhibitor and a member of the Cystatin superfamily. Cystatin C is widely expressed in all nucleated cells in the body. It is also secreted from cells into circulation and other biological fluids including urine, seminal fluid, saliva and cerebrospinal fluid. Cystatin C specifically inhibits cysteine proteases such as papain, legumain and the cathepsins B, H, K, L and S. Through the regulation of cysteine proteases, it plays a role in various biological processes including immune responses, protein turnover, phatocytosis, antigen presentation and apoptosis. When released into the blood, cystatin C is filtered by the glomerous and metabolised by proximal tubular cells. Because of this filtration of cystatin C by the kidneys, this inhibitor is commonly used clinically as a biomarker of kidney function. Within immunological processes, cystatin C regulates proteolytic processing of pro-granzymes, MHC II antigen presentation and maturation of dendritic cells. Cystatin C is encoded by the gene CST3 and mutations of this gene are responsible for the heritable disease, cerebral amyloid angiopathy. The purified Mouse anti Human cystatin C antibody, clone B01-8B1 (MCA6226GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human cystatin C antibody, clone E01-8H8 (MCA6227B) as the detection antibody.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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