Anti-Clostridium difficile Toxin A Antibody [253/422]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Clostridium difficile Toxin A

Applications

ELISA, IF

Reactivity

Clostridium difficile

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

ELISA: 1-5 µg/ml, IF: 1-2 µg/ml

Molecular weight

308 kDa (Toxin A)

Light chain

kappa

Clonă

253/422

Imunogen

C. difficile Toxin A.

Specificitate

This antibody reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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