Anti-CD86 Antibody [PO3] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD86

Applications

Flow Cytometry

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Clonă

PO3

Imunogen

Mouse B-cell line, BCL1.

Specificitate

This antibody recognises mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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