Anti-CD81 Antibody [Eat2]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 100µg · 250µg

Țintă

CD81

Applications

WB, IP, Flow Cytometry, IHC-Fr, ELISA

Reactivity

Mouse, Rat

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Hamster

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:10 for 0.1 mg, 1:50 for 0.25 mg, 25 µg - 1:50 for 0.1 mg, 1:100 for 0.25 mg, 25 µg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Clonă

Eat2

Imunogen

38C13, murine B cell line.

Specificitate

This antibody recognises mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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