Anti-CD68 Antibody [ED1] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD68

Applications

Flow Cytometry

Reactivity

Rat, Bovine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

FC 1: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Cross reactivity

This antibody does not cross react with Horse.

Clonă

ED1

Imunogen

Rat spleen cells.

Specificitate

This antibody recognises the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface. The expression of ED1 antigen being predominantly cytoplasmic , flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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