Anti-CD63 Antibody [AD1]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 100µg

Țintă

CD63

Applications

WB, IHC-P, IP, Flow Cytometry

Reactivity

Rat

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:10 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

AD1

Imunogen

RBL-2H3 rat basophilic leukaemia cell line.

Specificitate

This antibody recognises rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell types in vitro. Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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