Anti-CD62L Antibody [FMC46] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 100µg

Țintă

CD62L

Applications

Flow Cytometry

Reactivity

Human, Bovine, Cynomolgus monkey, Rhesus Monkey, Dog

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: 1:25 - Neat Pack Size: 0.1 mg 1:100 Pack Size: 25 µg, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Clonă

FMC46

Imunogen

PHA stimulated lymphoblasts.

Specificitate

This antibody recognises human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes.CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains.The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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